Anti-RXR alpha (RXRA, NR2B1, retinoid X receptor, alpha)

Applications: , Suitable for use in Peptide ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.3-1ug/ml. Approx 60kD band observed in nuclear lysates of cell line HeLa (calculated MW of 50.8kD according to NP_002948). , Optimal dilutions to be determined by the researcher. Titer: Peptide ELISA: antibody detection limit dilution 1:32,000. Control Peptide: R9985-02-P, Peptide Blocking: To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.