Anti-RSPO4 / R-spondin-4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The RSPO4 antibody targets R-spondin-4, a secreted growth factor encoded by the RSPO4 gene that acts as a potent activator of Wnt/beta-catenin signaling. R-spondin-4 belongs to the R-spondin family (RSPO1-4), which enhances Wnt receptor complex stability and signaling intensity through interactions with LGR4-6 receptors and the transmembrane E3 ubiquitin ligases RNF43 and ZNRF3. By amplifying Wnt responses, R-spondin-4 plays key roles in epithelial development, tissue repair, and organogenesis. The RSPO4 antibody enables precise detection of this signaling enhancer and supports research into developmental biology and regenerative medicine.R-spondin-4 is characterized by two cysteine-rich furin-like domains that mediate receptor binding and a thrombospondin type 1 (TSP1) domain that influences protein localization. Through these domains, R-spondin-4 stabilizes Frizzled and LRP5/6 receptors on the cell surface, prolonging Wnt pathway activation. The RSPO4 antibody helps researchers examine this process by detecting protein expression in tissues where Wnt signaling drives growth and differentiation, including skin, limb, and nail matrix.Mutations in RSPO4 cause anonychia congenita, a rare developmental disorder marked by complete or partial absence of nails. Such mutations impair Wnt pathway activation, underscoring R-spondin-4's essential role in ectodermal appendage development. The RSPO4 antibody is invaluable for exploring these mechanisms, allowing measurement of protein levels and localization in affected tissues. In experimental systems, recombinant R-spondin-4 is used to stimulate stem cell proliferation and differentiation, emphasizing its relevance in tissue engineering and regenerative therapies.Beyond development, R-spondin-4 has been implicated in tumorigenesis, particularly in cancers exhibiting aberrant Wnt signaling such as colorectal and gastric cancer. The RSPO4 antibody allows assessment of expression patterns in tumor samples and helps determine how altered RSPO4 activity contributes to cancer cell proliferation. In stem cell biology, this protein functions as a niche signal that maintains progenitor cell populations, making it a target of interest in organoid culture and tissue regeneration research.The RSPO4 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields strong staining of secreted and extracellular matrix-associated R-spondin-4. NSJ Bioreagents provides this antibody as a high-quality reagent for reproducible use in molecular, developmental, and cancer biology. By enabling detailed study of R-spondin-4 expression and signaling, the RSPO4 antibody supports discovery into Wnt pathway modulation and its implications for growth, regeneration, and disease.