Anti-RPS21 / 40S ribosomal protein S21

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. RPS21 antibody detects 40S ribosomal protein S21, encoded by the RPS21 gene on chromosome 20p11.23. RPS21 antibody is used in studies of ribosome assembly, translation, and protein synthesis regulation. RPS21 is a component of the small ribosomal subunit (40S) and contributes to decoding mRNA and ensuring fidelity of translation. Expression is ubiquitous across tissues, reflecting the essential requirement for ribosomal proteins in cell growth and proliferation. Mutations in ribosomal protein genes, including RPS21, are associated with ribosomopathies and cancer.Structurally, RPS21 is a small basic protein that integrates into the head region of the 40S ribosomal subunit. It contributes to the architecture of the decoding center and interacts with ribosomal RNA and other ribosomal proteins. Structural studies place RPS21 near the mRNA entry channel, supporting its role in guiding and stabilizing mRNA during translation. It is encoded by a highly conserved gene, underscoring its fundamental role in ribosome function.Functionally, RPS21 ensures proper initiation and elongation of protein synthesis. By contributing to ribosomal stability, it influences translation efficiency and fidelity. Knockdown of RPS21 reduces global protein synthesis and triggers nucleolar stress responses. Deficiency activates p53 pathways and can impair cell proliferation, linking RPS21 to cell cycle control. Researchers employ RPS21 antibody to study ribosome biogenesis, translational control, and stress responses.Clinically, RPS21 mutations have been identified in Diamond-Blackfan anemia, a congenital ribosomopathy characterized by bone marrow failure and developmental abnormalities. Somatic alterations in RPS21 expression are also observed in cancers, particularly hematologic malignancies, where dysregulated protein synthesis supports tumor growth. Elevated expression correlates with poor prognosis in some tumors. NSJ Bioreagents provides RPS21 antibody to facilitate research into ribosome biology, disease mechanisms, and translational regulation.Experimentally, RPS21 antibody is used in western blotting to detect the ~9 kDa protein, in immunohistochemistry to study nucleolar expression, and in immunofluorescence microscopy to localize it within ribosomes. Immunoprecipitation with RPS21 antibody enables analysis of ribosomal complexes and translation machinery.