Anti-rod-1

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis (PubMed:11590237, PubMed:18765790). Required for chromosome segregation, the assembly of the dynein-dynactin and mdf-1-mdf-2 complexes onto kinetochores and spindle pole separation (PubMed:18765790, PubMed:19109417). Plays a role in nuclear envelope breakdown (PubMed:19109417). Its function related to the spindle assembly machinery and kinetochore-microtubule attachments likely depends on its association in the mitotic RZZ complex (PubMed:18765790, PubMed:24231804). The RZZ complex recruits the spindly-like protein spdl-1 to kinetochores (PubMed:18765790, PubMed:24231804). To prevent irregular chromosome segregation, the complex also inhibits the attachment of the kinetochore-associated NDC80 complex to microtubules (PubMed:24231804). The recruitment of spdl-1 to kinetochores relieves this inhibition (PubMed:24231804). Required for embryonic development (PubMed:11590237, PubMed:18765790, PubMed:19109417). [The UniProt Consortium]