Anti-Ring Finger Protein 7 (RNF7, CKII beta-binding Protein 1, CKBBP1, Regulator of Cullins 2, ROC2,

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:8000, Western Blot: 1-3ug/ml ~11kD band observed in Human Human Heart lysates (calculated MW of 12.7kD according to NP_055060.1)., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.