Anti-RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58

RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (paraffin): 5ug/ml detects a band at ~84kD in C2C12 cell lysate. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6, is recommended., Western Blot: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human heart , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ,