Anti-RhCG

developmental stage:Specifically expressed in fetal kidney.,function:Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.,PTM:N-glycosylated.,similarity:Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.,subcellular location:Also detected at the basolateral membrane and in subapical vesicles.,tissue specificity:Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). Specifically expressed in kidney according to PubMed:11062476., Protein function: Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. Postulated to primarily mediate an electroneutral bidirectional transport of NH3 ammonia species according to a mechanism that implies interaction of an NH4(+) ion with acidic residues of the pore entry followed by dissociation of NH4(+) into NH3 and H(+). As a result NH3 transits through the central pore and is protonated on the extracellular side reforming NH4(+) (PubMed:11062476, PubMed:14761968, PubMed:15929723, PubMed:16477434, PubMed:16580862, PubMed:24077989). May act as a CO2 channel providing for renal acid secretion (PubMed:24077989). [The UniProt Consortium] Recommended dilutions: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.. Cellular localization: Apical cell membrane , Multi-pass membrane protein . Also detected at the basolateral membrane and in subapical vesicles. .