Anti-RGS8 (Regulator of G-protein Signaling 8)

RGS8 is a 22-23kD (predicted) member of the B/R4 subfamily, RGS family of proteins. It is expressed in NK cells and neurons, particularly cerebellar Purkinje cells, and is known to regulate GPCR signaling. When GPCRs are activated, a GPCR cytoplasmic domain associated GABG heterotrimer dissociates in response to GTP binding. This generates GA :GTP and GBG dimers that initiate downstream signaling. GA has GTPase activity, and when GTP is hydrolyzed, the trimer reforms and stops signaling. RGS8 potentiates GA GTP hydrolase activity, particularly when associated with M1 muscarinic acetylcholine receptors. Human RGS8 is 180aa in length, and is characterized by the presence of one RGS domain aa56-171, and an N-terminal GPCR binding motif aa6-9. There is one splice variant that shows a 27aa substitution for aa1-9. Expression of this variant may negatively impact its regulation of GA-coupled responses. Over aa10-76, human RGS8 shares 98% aa identity with mouse RGS8. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.