Anti-REST / RE1-silencing transcription factor

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. REST antibody detects RE1-silencing transcription factor, a zinc finger DNA-binding protein that represses neuronal gene expression in non-neuronal cells. The UniProt recommended name is RE1-silencing transcription factor (REST). Also known as neuron-restrictive silencer factor (NRSF), this protein plays a pivotal role in epigenetic regulation of gene expression by binding to RE1/NRSE motifs and recruiting chromatin-modifying complexes.Functionally, REST antibody identifies a 1097-amino-acid nuclear protein that suppresses transcription by forming repressive chromatin structures. REST recognizes RE1 sites present in the promoters of neuronal genes and recruits corepressors such as CoREST, mSin3, and histone deacetylases (HDAC1/2). These interactions promote chromatin condensation and transcriptional silencing, preventing inappropriate neuronal gene expression in non-neuronal tissues.The REST gene is located on chromosome 4q12 and encodes a multi-zinc finger transcription factor with eight C2H2 zinc finger motifs that mediate sequence-specific DNA binding. REST acts as a master regulator of neurogenesis, ensuring proper timing of neuronal differentiation. During development, REST levels decline in differentiating neurons, allowing the expression of genes required for synaptic function, ion channel activity, and neurotransmitter release.In addition to developmental regulation, REST participates in neural plasticity, stem cell maintenance, and stress response pathways. Dysregulation of REST has been linked to neurological disorders, including epilepsy, Huntington's disease, and Alzheimer's disease. Elevated REST expression in non-neuronal cancers such as medulloblastoma and neuroblastoma suggests a role in tumorigenesis through repression of differentiation pathways.REST antibody is widely used in neuroscience, epigenetics, and cancer research. It is suitable for chromatin immunoprecipitation (ChIP), immunoblotting, and immunofluorescence to study REST localization, DNA binding, and interaction networks. This antibody aids in exploring transcriptional silencing mechanisms and neurodevelopmental regulation. In oncology, REST serves as a biomarker of altered differentiation and epigenetic reprogramming.Structurally, REST contains an N-terminal repression domain that recruits mSin3-HDAC complexes and a C-terminal domain that binds CoREST and associated enzymes such as LSD1. REST-mediated repression involves coordinated histone deacetylation and demethylation, establishing a transcriptionally repressive chromatin environment. NSJ Bioreagents provides REST antibody reagents validated for use in transcriptional repression, neuronal gene regulation, and chromatin remodeling studies.