Anti-Receptor Interacting Protein 140 (RIP140, RIP-140, Nuclear Factor RIP-140, Nuclear Factor RIP14

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor-Interacting Protein 140, also known as RIP140, has been shown to enhance estrogen receptor transcriptional activity through the AF2-AD domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPARa) and has been proposed to competitively inhibit PPARa interaction with another known nuclear receptor coactivator, SRC-1. However, the specific roles for coactivation and corepression by RIP140 in steroid receptor pathways is currently not well understood. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.