Anti-RB

The Rb protein regulates differentiation, apoptosis, and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser-795 marks the conversion of Rb from a transcriptionally repressive hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene (Rb). Applications: Suitable for use in ELISA ans Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000, Immunohistochemistry: 1:200-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.