Anti-RASSF8 / Ras association domain-containing protein 8

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. RASSF8 antibody detects Ras association domain-containing protein 8, a cytoplasmic and membrane-associated protein involved in cell adhesion, cytoskeletal organization, and tumor suppression. The UniProt recommended name is Ras association domain-containing protein 8 (RASSF8). This member of the Ras association domain family links small GTPase signaling to cytoskeletal remodeling and intercellular junction stability.Functionally, RASSF8 antibody identifies a 413-amino-acid protein containing an RA (Ras association) domain and a coiled-coil region that mediates homo- and hetero-oligomerization. RASSF8 localizes to adherens junctions and the plasma membrane, where it interacts with proteins such as E-cadherin, beta-catenin, and SCRIB. Through these interactions, RASSF8 supports cell polarity and epithelial integrity by regulating actin cytoskeleton organization. It may also participate in Ras-related signaling pathways that influence apoptosis and cell migration.The RASSF8 gene is located on chromosome 12p12.1 and is expressed in epithelial and endothelial tissues, with high expression in lung, colon, and skin. It acts downstream of Ras and Rap1A, functioning as a molecular adaptor to relay signals from membrane GTPases to cytoskeletal and junctional complexes. RASSF8 expression is dynamically regulated during epithelial differentiation and stress responses.Pathologically, loss or reduced expression of RASSF8 is associated with various cancers, including lung, colorectal, and breast carcinoma. Its downregulation disrupts adherens junctions, promotes epithelial-mesenchymal transition (EMT), and enhances tumor cell invasion. RASSF8 may act as a tumor suppressor by maintaining cell adhesion and suppressing uncontrolled proliferation. Research using RASSF8 antibody supports studies in cancer biology, cell adhesion, and epithelial polarity regulation.RASSF8 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect cytoskeletal and adhesion-related proteins. NSJ Bioreagents provides RASSF8 antibody reagents optimized for studies in Ras signaling, cell polarity maintenance, and epithelial junction assembly.Structurally, Ras association domain-containing protein 8 contains an RA domain that binds small GTPases and a C-terminal coiled-coil domain involved in multimerization and membrane targeting. This architecture allows RASSF8 to integrate Ras-related signaling with cytoskeletal remodeling. This antibody facilitates research into RASSF8's role in tumor suppression, cell adhesion, and cytoskeletal integrity.