Anti-RANBP3 / Ran-binding protein 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. RANBP3 antibody detects Ran-binding protein 3, a nuclear transport factor that regulates nucleocytoplasmic trafficking and RNA export. The UniProt recommended name is Ran-binding protein 3 (RANBP3). This protein functions as a cofactor in the Ran GTPase cycle, controlling the directionality of transport across the nuclear pore complex (NPC). RANBP3 is a crucial mediator in the export of proteins and RNAs from the nucleus to the cytoplasm, contributing to overall genome expression and cellular homeostasis.Functionally, RANBP3 antibody identifies a 607-amino-acid cytoplasmic and nuclear protein that interacts with Ran GTPase, CRM1 (also known as Exportin 1), and other nucleocytoplasmic transport components. RANBP3 facilitates CRM1-dependent export of cargo molecules by stabilizing the export complex in a RanGTP-bound state. It also acts as a cofactor promoting efficient release of exported cargo upon GTP hydrolysis. Through these functions, RANBP3 ensures proper distribution of transcription factors, mRNA, and ribonucleoprotein complexes between cellular compartments.The RANBP3 gene is located on chromosome 19p13.3 and is ubiquitously expressed across mammalian tissues. It contains an N-terminal nuclear export signal, a Ran-binding domain, and multiple leucine-rich motifs responsible for cargo recognition. RANBP3 participates in nuclear export of Smad proteins, linking it directly to TGF-beta signaling. By facilitating Smad nuclear export, it modulates transcriptional responses to growth factors and cytokines. Its activity is regulated by phosphorylation and nuclear-cytoplasmic shuttling that respond to cell cycle and signaling cues.In addition to its export functions, RANBP3 maintains genome integrity by controlling the localization of DNA repair factors and replication regulators. It also contributes to ribosome biogenesis and mRNA surveillance by interacting with nuclear export adaptors. Dysregulation of RANBP3 has been associated with tumor progression and antiviral defense, as viruses often hijack CRM1-dependent pathways to export viral RNA and proteins.RANBP3 antibody is widely used in studies of nucleocytoplasmic transport, gene regulation, and signal transduction. It is suitable for immunoblotting, immunofluorescence, and nuclear fractionation assays to examine RANBP3 distribution and function. This antibody supports research into RNA export, TGF-beta signaling, and nuclear pore dynamics. In disease studies, RANBP3 detection helps elucidate altered nuclear transport mechanisms contributing to cancer and viral infection.Structurally, RANBP3 contains a pleckstrin homology-like domain and a Ran-binding domain that coordinate GTPase activity and CRM1 interaction. Post-translational modifications such as phosphorylation at specific serine residues regulate its subcellular localization and export efficiency. NSJ Bioreagents provides RANBP3 antibody reagents validated for use in nucleocytoplasmic transport, transcriptional regulation, and signal transduction research.