Anti-RAMP2 (Receptor Activity Modifying Protein2)

Human RAMP2 (receptor activity modifying protein 2) is a 17kD member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature human RAMP2 is a 133aa type I transmembrane glycoprotein that contains a 103aa extracellular domain (ECD) (aa43-145) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa86-92 are critical for AM binding. There is one potential splice variant that shows a five aa insertion after Glu54. Over aa43-145, human RAMP2 shares 61% aa identity with mouse RAMP2. Members of RAMP family of proteins are known to form complexes with apparent molecular weight of 25kD to 50kD that are resistant to denaturing and reducing agents. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.