Anti-RAMP2 (Receptor Activity Modifying Protein2)

RAMP2 is a 20kD member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145aa type I transmembrane glycoprotein that contains a 115aa extracellular domain (ECD) aa45-159 and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa100-106 are critical for AM binding. Over aa45-159, mouse RAMP2 shares 57-83aa identity with human and rat RAMP2, respectively. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells , Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.