Anti-RAMP1 (Receptor Activity Modifying Protein1, Receptor Activity Modifying Protein 1 [Precursor],

RAMP1 (receptor activity modifying protein 1) is a 14-18kD member of the RAMP family of proteins. Variability in MW is due to the absence, or presence, of intramolecular disulfide bonds that form in the ER and Golgi. It is expressed on/in neurons, vascular endothelial cells, visceral and vascular smooth muscle cells, mammary epithelium, osteoblasts and cardiomyocytes. RAMP1 interacts with CRLR/CLR to form an 84kD noncovalent receptor complex for CGRP and AM, and with CTR to form a 76kD receptor complex for amylin. Mature human RAMP1 is a 122 aa nonglycosylated type I transmembrane protein that contains a 91aa extracellular domain (ECD) (aa27-117) plus a ten aa cytoplasmic tail. In the ECD, residues 78-90 bind AM, while residues 91-103 bind CGRP. In the absence of CRLR and CTR,RAMP1 will form 30-32kD disulfide-linked homodimers in the ER/Golgi. Over (aa27-117), human RAMP1 shares 65% aa identity with mouse RAMP1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.