Anti-Raf-1, clone 3H274

Applications: , Suitable for use in ELISA and Western Blot. Not suitable for detection of Raf-1 expression and phosphorylation in HeLa cells. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml will allow visualization of 0.2ug/lane of human Raf-1. A main band (75kD) was visible , Optimal dilutons to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.