Anti-RAB34 / Ras-related protein Rab-34

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. RAB34 antibody detects Ras-related protein Rab-34, a small GTPase involved in vesicular trafficking, lysosome positioning, and Golgi organization. The UniProt recommended name is Ras-related protein Rab-34 (RAB34). This protein functions as a molecular switch cycling between GTP-bound active and GDP-bound inactive states, controlling membrane dynamics and cargo transport in endocytic and secretory pathways.Functionally, RAB34 antibody identifies a 259-amino-acid cytosolic protein anchored to the Golgi apparatus through its C-terminal prenylation motif. In its GTP-bound state, RAB34 recruits effector proteins that regulate vesicle budding, movement, and fusion. It plays a key role in positioning lysosomes near the perinuclear region by interacting with the motor protein RILP and the lysosomal GTPase RAB7A. Through this mechanism, RAB34 coordinates lysosomal trafficking and degradation of cellular cargo.The RAB34 gene is located on chromosome 17q11.2 and is expressed in multiple tissues, including brain, liver, and epithelial cells. Its activity supports vesicular transport processes essential for endosome maturation and Golgi integrity. RAB34 also contributes to the formation of ruffled plasma membranes and macropinosomes, thereby facilitating nutrient uptake and receptor internalization. In the Golgi network, it assists in maintaining cisternal stacking and trafficking of glycoproteins to the plasma membrane.In cell signaling, RAB34 regulates the transport of signaling receptors such as EGFR and PDGFR, influencing downstream MAPK and AKT pathway activation. Dysregulation of RAB34 expression has been linked to cancer progression, where it affects migration, invasion, and exosome secretion. Elevated RAB34 expression correlates with poor prognosis in several cancers, including glioma, pancreatic carcinoma, and hepatocellular carcinoma, suggesting a role in tumor metastasis and lysosome-mediated proteolysis.RAB34 antibody is commonly used in cell biology, vesicular transport, and cancer research. It is suitable for immunoblotting, immunofluorescence, and co-localization studies to visualize Golgi and lysosomal compartments. This antibody enables detailed examination of RAB34 function in intracellular trafficking, receptor recycling, and organelle positioning. Its detection aids in exploring the role of RAB GTPases in membrane transport and signal regulation.Structurally, RAB34 shares the conserved GTP-binding motifs found in the RAS superfamily and undergoes prenylation at its C-terminal CAAX box for membrane anchoring. It cycles between active and inactive states under the control of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). NSJ Bioreagents provides RAB34 antibody reagents validated for use in vesicle trafficking, Golgi organization, and tumor biology research.