Anti-PYCR3 / Pyrroline-5-carboxylate reductase 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PYCR3 antibody detects Pyrroline-5-carboxylate reductase 3, a mitochondrial enzyme that catalyzes the NADH-dependent reduction of pyrroline-5-carboxylate to proline, completing the final step of proline biosynthesis. PYCR3 plays a key role in amino acid metabolism, redox regulation, and cell growth. The PYCR3 antibody is widely used in cancer metabolism, mitochondrial biology, and redox signaling research to study proline synthesis and metabolic adaptation.PYCR3 is encoded by the PYCR3 gene located on human chromosome 8q24.3. The protein is approximately 319 amino acids long and localizes to the mitochondrial matrix, functioning alongside PYCR1 and PYCR2 in the proline biosynthetic pathway. PYCR3 preferentially uses NADH as a cofactor and contributes to redox homeostasis by balancing NAD+/NADH ratios within mitochondria.The PYCR3 antibody detects a 33 kilodalton band by western blot and shows punctate mitochondrial localization under fluorescence microscopy. Through its regulation of proline metabolism, PYCR3 supports anabolic growth, oxidative stress resistance, and survival under nutrient deprivation. It also influences collagen synthesis and extracellular matrix remodeling by controlling intracellular proline availability.PYCR3 expression is upregulated in several cancers, including colorectal, lung, and prostate carcinoma, where it promotes tumor cell proliferation and metastasis by maintaining mitochondrial NADH pools. Knockdown of PYCR3 reduces ATP production, increases reactive oxygen species, and triggers apoptosis, demonstrating its role in metabolic flexibility. Outside of cancer, PYCR3 supports fibroblast function and tissue repair by providing proline for collagen biosynthesis.As a regulator of both amino acid metabolism and mitochondrial redox balance, PYCR3 represents a metabolic control point linking biosynthesis and energy maintenance. NSJ Bioreagents provides a validated PYCR3 antibody optimized for western blot, immunofluorescence, and mitochondrial assays, supporting research into amino acid metabolism, redox biology, and cancer bioenergetics.