Anti-PTPRN (IA-2, Insulinoma-assocated Protein 2, IA2, ICA512, R-PTP-N)

IA-2 (insulinoma­assocated protein 2), also called PTPRN (protein tyrosine phosphatase-like N) and ICA512 (islet cell antigen 512), is a 130kD member of the receptor tyrosine phosphatase family of type I transmembrane enzymes. It is not active as a phosphatase but acts an autoantigen in type I (insulin-dependent) diabetes. It is mainly expressed in neuroendocrine cells types within pancreatic islets, adrenal medulla, pituitary, and the central nervous system, and is present within membranes of regulated secretory granules. It is induced by glucose and insulin in the pancreas and is thought to contribute to the growth of beta cells. Cleavage at a furin-like site at amino acid (aa) 448 creates a 60-66kD transmembrane fragment. Within the region used as an immunogen, human IA-2 shares 80% aa sequence identity with mouse and rat IA-2. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 8-25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.