Anti-PSME4 / Proteasome activator complex subunit 4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PSME4 antibody detects Proteasome activator complex subunit 4, also known as PA200, a nuclear protein that regulates proteasome function during DNA repair and spermatogenesis. PSME4 binds to the 20S proteasome core to enhance peptide cleavage and modulate substrate selectivity in a ubiquitin-independent manner. The PSME4 antibody is widely used in proteostasis, cell cycle, and DNA repair research to study proteasome regulation and nuclear protein degradation.PSME4 is encoded by the PSME4 gene located on human chromosome 2q37.1. The protein is approximately 1,840 amino acids long and forms a ring-like structure that caps one end of the 20S proteasome. PSME4-mediated activation alters proteasome activity toward oxidized and acetylated substrates, enhancing degradation of histones and DNA repair factors following genotoxic stress.The PSME4 antibody detects a 220 kilodalton protein by western blot and shows strong nuclear localization under immunofluorescence microscopy. PSME4 plays a crucial role in DNA double-strand break repair by facilitating turnover of repair-associated chromatin proteins, including histones and Ku70/80. In male germ cells, PSME4 regulates histone replacement during spermatogenesis, ensuring chromatin condensation and sperm maturation.Deficiency of PSME4 leads to impaired proteasome activation and accumulation of damaged proteins, resulting in genomic instability and infertility. PSME4 also influences transcriptional regulation and aging by modulating histone acetylation and chromatin dynamics. In cancer, aberrant PSME4 expression alters proteasome activity, contributing to resistance against apoptosis and therapeutic stress.As a specialized proteasome activator, PSME4 provides insight into the noncanonical regulation of proteolysis and DNA repair. NSJ Bioreagents provides a validated PSME4 antibody optimized for its applications, supporting research into nuclear proteasome regulation, DNA damage response, and reproductive biology.