Anti-PSME3 / Proteasome activator complex subunit 3

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PSME3 antibody detects Proteasome activator complex subunit 3 (also known as PA28 gamma or REG gamma), a nuclear proteasome regulator involved in ubiquitin-independent protein degradation, cell cycle control, and stress responses. Encoded by the PSME3 gene on chromosome 17q21.2, this protein belongs to the 11S (PA28) family of proteasome activators that modulate the 20S core particle. Unlike its cytoplasmic counterparts PA28 alpha and beta, which form the PA28 alpha/beta complex activated by interferon-gamma, PSME3 functions mainly within the nucleus, where it regulates the turnover of transcriptional regulators, cyclins, and damaged proteins in a ubiquitin-independent manner.PSME3 forms homoheptameric rings that cap the 20S proteasome, enhancing peptide entry and release. This activation facilitates the degradation of short-lived or misfolded proteins without requiring ubiquitination or ATP. The protein is dynamically regulated through phosphorylation and interaction with other nuclear components, enabling rapid responses to cell stress or metabolic shifts. Key substrates of PSME3 include p21, p53, and steroid receptor coactivators, indicating its dual role in proteostasis and transcriptional regulation.The PSME3 antibody is widely used in cell biology, oncology, and proteasome regulation research to assess nuclear proteasome activity and protein turnover. Western blot analysis identifies a 29 kilodalton band corresponding to PA28 gamma, and immunofluorescence reveals diffuse nuclear staining. This antibody supports studies of proteolytic regulation in proliferation, apoptosis, and DNA damage responses. Because PSME3 controls the degradation of regulatory proteins involved in the cell cycle and tumor suppression, its dysfunction is associated with oncogenesis and altered stress tolerance.In cancer, overexpression of PSME3 promotes tumor progression by accelerating degradation of p53 and p21, thereby weakening cell-cycle checkpoints and apoptosis. Conversely, loss of PSME3 function leads to accumulation of damaged proteins and proteostasis stress. Beyond cancer, PSME3 contributes to chromatin regulation, immune surveillance, and viral infection control. The PSME3 antibody enables researchers to examine these diverse processes by providing reliable detection in western blot, immunofluorescence, and immunohistochemistry. NSJ Bioreagents offers this antibody validated for high specificity in proteasome and nuclear turnover studies.