Anti-PSMC5 / 26S proteasome regulatory subunit 8, clone 29P84

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. PSMC5 antibody detects 26S proteasome regulatory subunit 8, encoded by the PSMC5 gene. This protein is one of the six ATPases of the AAA family that form the base of the 19S regulatory particle of the proteasome. PSMC5, also known as TBP1, provides ATP hydrolysis required for unfolding and translocating ubiquitinated substrates into the 20S core particle for degradation. PSMC5 antibody provides researchers with a reliable tool to study protein homeostasis, cell cycle regulation, and signaling pathways dependent on proteasomal degradation.The 26S proteasome is responsible for the majority of regulated intracellular protein degradation in eukaryotic cells. By removing misfolded proteins, damaged proteins, and short lived regulatory proteins, the proteasome maintains proteostasis and controls key signaling events. Research using PSMC5 antibody has shown that 26S proteasome regulatory subunit 8 contributes to ATP driven unfolding, ensuring substrate entry into the proteolytic chamber. This function is essential for turnover of cell cycle regulators, transcription factors, and signal transduction intermediates.Dysfunction of the proteasome contributes to numerous diseases, including neurodegenerative disorders such as Parkinson and Alzheimer disease, where accumulation of protein aggregates is a hallmark. Research with PSMC5 antibody has demonstrated that altered proteasome activity leads to impaired clearance of aggregation prone proteins. In cancer, hyperactivation of the proteasome allows degradation of tumor suppressors and cell cycle inhibitors, promoting unchecked proliferation. PSMC5 has also been implicated in immune regulation, where proteasomal processing generates antigenic peptides presented on MHC class I molecules.PSMC5 antibody is widely used in western blotting, immunoprecipitation, and immunohistochemistry. Western blotting demonstrates abundance in metabolically active tissues, while immunoprecipitation allows isolation of 19S regulatory complexes containing PSMC5. Immunohistochemistry highlights proteasome distribution in brain, muscle, and immune organs. These applications make PSMC5 antibody versatile in both fundamental and translational research.By supplying validated PSMC5 antibody reagents, NSJ Bioreagents supports research into proteasome biology, neurodegeneration, and oncology. Detection of 26S proteasome regulatory subunit 8 ensures that researchers can examine how protein degradation maintains cellular health and contributes to disease when dysregulated.