Anti-PSF/SFPQ (IHC)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
IHC-00304-T 10 µl (5+ slides) -

3 - 9 business days

125.00€
IHC-00304 100 µl (50+ slides) -

3 - 9 business days

470.00€
 
Protein function: DNA- and RNA binding protein, involved in several nuclear processes. Essential... more
Product information "Anti-PSF/SFPQ (IHC)"
Protein function: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells, T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. [The UniProt Consortium]
Keywords: Anti-PSF, Anti-SFPQ, Anti-hPOMp100, Anti-100 kDa DNA-pairing protein, Anti-PTB-associated-splicing factor, Anti-Splicing factor, proline- and glutamine-rich, Anti-DNA-binding p52/p100 complex, 100 kDa subunit
Supplier: Bethyl Laboratories
Supplier-Nr: IHC-00304-T

Properties

Application: IHC, IHC-IF
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Mouse (Expected: Rat, X. Tropicalis, Chicken, Turkey, Bovine, Dog, Horse, Rabbit, Guinea Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Tasmanian Devil, White-tufted-ear Marmoset, Northern White-cheeked Gibbon)
Immunogen: synthetic peptide. The epitope recognized by IHC-00304 maps to a region between residue 657 and 707 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutamine-rich) using the numbering given in entry NP_005057.1 (GeneID
Format: Antigen Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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