Anti-Protein Kinase C (PKC)

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot (ECL): 2ug/ml will detect cPKC in 20ug of rat brain tissue extract., Immunoprecipitation: 12.5ug/ml, Immunohistochemistry: 1:50, Optimal dilutions to be determined by researcher. Positive Controls: Mouse Brain Tissue Extract and Rat Brain Tissue Extract, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.