Anti-Protein Kinase C, iota, lambda, phosphorylated (Thr555/Thr563) (PKCi, PKC, Atypical Protein Kin

Protein Kinase C, iota (PKCi) is a 76kD member of the atypical group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. Phosphorylation of PKCi plays a key role in protein trafficking. Phosphorylated PKCi is implicated in high glucose-induced T type II transforming growth factor-beta activity, TNF-a-stimulated NF-kB activation and Bcr/Ablmediated anti-apoptotic effect. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells treated with PMA, a phorbol ester. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.