Anti-Protein C Receptor, Endothelial (EPCR, PROCR)

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to the Endothelial Protein C Receptor (EPCR) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rmEPCR is ~ 3ng/well., Western Blot: 1-2ug/ml detection limit for rmEPCR is~ 50ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.