Anti-PROS1 (Protein S)

Protein S (PROS1) is a 70­75kD plasma glycoprotein member of the vitamin K dependent family of proteins. It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium. Protein S is a non­enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa. It also binds to Axl on smooth muscle, likely initiating mitosis. Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP. Mouse Protein S precursor is 675 amino acids (aa) in length. It contains a signal sequence (aa1­24), a cleavable prosegment (aa25­41) and a 634aa mature region. The mature molecule contains one Gla region (aa42­87), four EGF­like domains (aa117­283) and two laminin G­like regions (aa299­665). Over aa1­169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.