Anti-Prohibitin

Prohibitin is believed to be a negative regulator of cell proliferation and may also act as a tumor suppressor. Mutations in prohibitin have been linked to sporadic breast cancer. Prohibitin inhibits DNA synthesis and plays a role in proliferation regulation. As yet, it is unclear if it is the protein or the mRNA that exhibits these effects. Prohibitin may play a role in regulating mitochondrial respiration activity and aging. Prohibitin is located at the inner mitochondrial membrane and is widely expressed in various tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.