Anti-Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3)

The progesterone receptor (PR) is a member of the superfamily of steroid hormone receptors. In response to ligand binding, these nuclear receptors bind to their target DNA response element and modulate the transcription of specific genes. Hormone induced binding of receptor to its response element promotes the formation of a stable initiation complex, which allows for efficient transcription by RNA polymerase II. Steroid hormone receptors are characterized by a six domain structure labeled A through F. The A/B domain is responsible for trans-activation, C is the DNA binding region and the most highly conserved, D is involved in nuclear localization and E has a variety of functions including ligand binding, dimerization, interaction with HSP90 and trans-activation. There are two forms of the progesterone receptor, the Areceptor (94kD) is a naturally occurring, truncated form of the B-receptor (120kD). Both forms bind progesterone but may have distinct biological properties. PR is expressed in several tissues especially uterus and breast. PR is one of the most important hormones in the regulation of growth and differentiation in mammary tissue and is thought to play an important role in mammary carcinogenesis. The measurement of PR has become an important prognostic factor in breast cancer and is useful for predicting the likelihood of response to endocrine therapy. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. T-47D cells can be used positive control. Recognizes the ~94kD progesterone receptor A and the ~120kD progesterone receptor B., ELISA: 1:10,000-1:50,000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.