Anti-PP1 beta (protein phosphatase 1, catalytic subunit, beta isoform, MGC3672, PP-1B, PPP1CD, Serin

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500 detected PP1 beta in 10ug of HeLa lysates, Jurkat lysates, RAW 264 lysates, HEK293 lysates, NIH/3T3 lysates, C2C12 lysates, PC12 lysates, A431 lysates and HepG2 lysates. , Immunohistochemistry (Paraffin): 1:100 detected PP1 beta in normal human testis. Tissue was pretreated with citrate buffer, pH 6.0. , Immunoprecipitation: 10-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.