Anti-POLR3D / DNA-directed RNA polymerase III subunit RPC4

0.5mg/ml if reconstituted with 0.2ml sterile DI water. POLR3D antibody is a key reagent for studying transcriptional regulation and RNA metabolism. The encoded protein, DNA-directed RNA polymerase III subunit RPC4, is an essential component of RNA polymerase III, the enzyme complex responsible for synthesizing small structural RNAs including tRNAs, 5S rRNA, and other short non-coding RNAs. These transcripts are fundamental to ribosome biogenesis, protein synthesis, and multiple regulatory pathways. By supporting the assembly and activity of RNA polymerase III, POLR3D ensures proper cellular growth, proliferation, and maintenance of homeostasis.DNA-directed RNA polymerase III subunit RPC4 contributes to the conserved catalytic core of the enzyme, providing structural stability and facilitating transcriptional activity. Its interactions with other polymerase subunits form part of the framework that enables RNA chain initiation and elongation. Beyond its basal role, POLR3D helps modulate transcription in response to signaling pathways, linking environmental and metabolic cues to RNA production.Mutations in DNA-directed RNA polymerase III subunit RPC4 have been associated with rare neurodevelopmental syndromes, underscoring its importance in human biology. Furthermore, RNA polymerase III and its subunits, including POLR3D, are closely tied to innate immune function. Viral infections often exploit RNA polymerase III activity, while host defenses detect RNA polymerase III products as part of antiviral recognition systems. This highlights the dual role of POLR3D in normal physiology and immune response.Cancer research has also implicated RNA polymerase III dysregulation, where increased transcription of tRNAs and 5S rRNA drives uncontrolled protein synthesis and cellular proliferation. As part of this complex, DNA-directed RNA polymerase III subunit RPC4 may contribute to tumor development by supporting excessive biosynthetic activity. Ongoing studies are examining whether POLR3D and its associated pathways can serve as biomarkers or therapeutic targets.The POLR3D antibody is applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to assess expression, localization, and changes in disease or experimental models. These tools are vital for researchers investigating transcriptional regulation, immune surveillance, and oncogenic pathways. For advanced molecular studies requiring consistent and accurate detection, the POLR3D antibody from NSJ Bioreagents provides dependable performance.