Anti-POLG2 / DNA polymerase subunit gamma-2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. POLG2 antibody detects DNA polymerase subunit gamma-2, a key accessory subunit of the mitochondrial DNA polymerase complex responsible for mitochondrial genome replication and repair. POLG2 enhances the processivity and stability of the catalytic subunit POLG, ensuring faithful replication of mitochondrial DNA (mtDNA). The POLG2 antibody is widely used in mitochondrial biology, genetics, and aging research to study DNA maintenance, oxidative stress, and mitochondrial dysfunction.POLG2 is encoded by the POLG2 gene located on human chromosome 17q23.3. The protein is approximately 485 amino acids in length and localizes to the mitochondrial matrix. It forms a homodimer that binds to POLG, increasing its affinity for DNA and reducing dissociation during replication. Through this interaction, POLG2 coordinates mtDNA replication and repair under both physiological and stress conditions.The POLG2 antibody detects a 55 kilodalton band by western blot and shows mitochondrial punctate staining under immunofluorescence microscopy. Loss-of-function mutations in POLG2 result in reduced mtDNA copy number, leading to multiple mitochondrial DNA deletions, oxidative stress, and mitochondrial depletion syndromes. Clinical manifestations include progressive external ophthalmoplegia, ataxia, and myopathy.POLG2 also contributes to mitochondrial biogenesis and interacts with nucleoid proteins such as TFAM and TWINKLE, supporting DNA packaging and maintenance. Dysregulation of POLG2 expression has been observed in neurodegenerative diseases, aging tissues, and metabolic disorders, where impaired mtDNA replication leads to reduced energy output and increased reactive oxygen species production.Because of its essential role in maintaining mitochondrial genome integrity, POLG2 serves as a critical factor in understanding mitochondrial pathophysiology and age-related dysfunction. NSJ Bioreagents provides a validated POLG2 antibody optimized for its applications, supporting research into DNA replication, repair, and mitochondrial disease mechanisms.