Anti-Podocalyxin (Podocalyxin-like Protein, PDXL)

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidne y development and homeostasis, blood filtration and urine formation. It is also expressed on va scular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturi a) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml, Flow Cytometry: 0.5-1ug/10e6 cells, Western Blot: 0.5-1ug/ml, Immunoprecipitation: 1-2ug/500ug protein, Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT, Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney , Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.