Anti-PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglycer

Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:8000. , Western Blot: 0.2-0.6ug/ml. A band of ~55kD observed in Human Adipose lysates (calculated MW of 55.3kD according to NP_065109.1). , Optimal dilutions to be determined by the researcher. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P4350-15C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ,