Anti-PNKP / Polynucleotide kinase phosphatase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PNKP antibody detects Polynucleotide kinase phosphatase, a DNA repair enzyme that restores damaged DNA termini during base excision and single-strand break repair. PNKP possesses both 5'-kinase and 3'-phosphatase activities, preparing DNA ends for ligation by DNA ligase. The PNKP antibody is widely used in DNA repair, neurobiology, and cancer research to study genome maintenance, oxidative damage response, and repair pathway coordination.PNKP is encoded by the PNKP gene on human chromosome 19q13.33. The protein is approximately 521 amino acids long and contains an N-terminal forkhead-associated (FHA) domain that mediates interactions with XRCC1 and XRCC4, scaffolding proteins in the single-strand and double-strand break repair complexes. The C-terminal catalytic region includes both phosphatase and kinase domains that process DNA termini with abnormal 3'-phosphate or 5'-hydroxyl ends.The PNKP antibody detects a 57 kilodalton band by western blot and shows nuclear foci following DNA damage. PNKP plays a dual role in the repair of oxidative and radiation-induced DNA breaks by preparing strand ends for gap filling and ligation. Mutations in PNKP cause neurological disorders such as microcephaly with early-onset seizures and developmental delay, resulting from defective DNA repair in postmitotic neurons.PNKP activity is tightly regulated by phosphorylation and interaction with repair complexes. In cancer, overexpression of PNKP enhances DNA repair capacity, contributing to therapy resistance. Pharmacologic inhibition of PNKP sensitizes tumor cells to radiation and chemotherapeutic agents, underscoring its potential as a therapeutic target.Because PNKP maintains genomic stability and neuronal survival, it serves as a key model for studying the interface between DNA repair and neurodegeneration. NSJ Bioreagents provides a validated PNKP antibody optimized for western blot, immunofluorescence, and DNA repair assays, supporting detailed analysis of genome maintenance and repair coordination.