Anti-PLS1 / Plastin-1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PLS1 antibody detects Plastin-1, an actin-bundling protein that stabilizes and crosslinks actin filaments in epithelial and sensory cells. Encoded by the PLS1 gene on chromosome 3q23, this protein belongs to the plastin family of actin-bundling proteins, which includes isoforms expressed in hematopoietic, ubiquitous, and epithelial tissues. Plastin-1 plays an essential structural role in maintaining brush border microvilli in the intestinal epithelium and stereocilia in the inner ear, contributing to cellular rigidity and signal transduction.Structurally, Plastin-1 is a 630-amino-acid cytoskeletal protein of approximately 68 kilodaltons containing two tandem calponin homology (CH) domains responsible for actin binding and two EF-hand calcium-binding motifs that regulate its activity. The protein localizes predominantly to actin-rich structures such as microvilli and adherens junctions, where it bundles filamentous actin into parallel arrays that reinforce cell shape and polarity.The PLS1 antibody is widely used in cell biology, auditory physiology, and epithelial research to study cytoskeletal architecture, microvillar dynamics, and actin filament regulation. Western blot analysis detects a 68 kilodalton band corresponding to Plastin-1, while immunofluorescence demonstrates strong staining along apical membranes in epithelial cells. This antibody provides a reliable tool for investigating actin organization and microvillar maintenance.Functionally, Plastin-1 is critical for maintaining the structural stability of brush border and sensory epithelia by linking actin filaments into tightly packed bundles. Mutations in PLS1 cause autosomal recessive nonsyndromic hearing loss due to disruption of stereocilia structure and function in cochlear hair cells. In intestinal cells, PLS1 supports nutrient absorption and epithelial resilience under mechanical stress. The PLS1 antibody supports studies of cytoskeletal integrity, epithelial morphogenesis, and hereditary hearing loss. NSJ Bioreagents validates this antibody for its applications, ensuring accurate detection of Plastin-1 in cytoskeletal research.