Anti-Phosphodiesterase 5A (PDE5A, cGMP-Specific 3',5'-Cyclic Phosphodiesterase, cGMP-Binding cGMP-Sp

Phosphodiesterase 5A, a cGMP-specific phosphodiesterase, has been implicated in primary pulmonary hypertension, cardiac failure and erectile dysfunction. PDE5A also may function in the regulation of coronary blood flow. PDE5A is the predominant enzyme responsible for cGMP hydrolysis in trabecular smooth muscle. PDE5A activitity is inhibited by sildenafil (Viagra) and zaprinast. At least three alternatively spliced transcripts have been reported. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP*, Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.