Anti-PHF8 / Plant homeodomain finger protein 8

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PHF8 antibody detects Plant homeodomain finger protein 8, a histone demethylase that regulates chromatin structure and transcription by removing methyl groups from lysine residues on histones. PHF8 is a member of the JmjC domain-containing family of histone demethylases and is particularly known for its ability to demethylate mono- and dimethylated histone H3 lysine 9 and lysine 27. Through this enzymatic activity, PHF8 influences gene activation during development, cell cycle progression, and neuronal differentiation. The PHF8 antibody is widely used in epigenetics and developmental biology research to study histone modification, chromatin remodeling, and transcriptional regulation.PHF8 is encoded by the PHF8 gene located on human chromosome Xp11.22. The protein is approximately 1,024 amino acids in length and contains two major domains: a plant homeodomain (PHD) finger that recognizes trimethylated histone H3 lysine 4 and a Jumonji C (JmjC) catalytic domain responsible for demethylation. By binding to active chromatin marks via the PHD domain, PHF8 is recruited to promoter regions where it removes repressive histone marks, facilitating transcriptional initiation.The PHF8 antibody detects a 120-140 kilodalton protein by western blot and shows nuclear localization under immunofluorescence microscopy. PHF8 participates in multiple developmental processes, including craniofacial morphogenesis and neural differentiation. In neurons, it regulates genes required for synaptic growth, neurite extension, and neurotransmission. Loss of PHF8 function leads to intellectual disability, cleft lip and palate, and X-linked mental retardation syndromes, emphasizing its critical role in human development.At the molecular level, PHF8 acts as both an epigenetic activator and repressor, depending on context. It associates with transcription factors such as TCF3 and ZNF711 to coordinate developmental gene expression programs. PHF8 also interacts with components of the RNA polymerase II complex, linking histone demethylation to active transcription elongation. In cancer biology, overexpression of PHF8 enhances cell proliferation and epithelial-to-mesenchymal transition, while its depletion suppresses tumor growth by re-establishing chromatin repression.Because of its dual functions in transcriptional activation and chromatin organization, PHF8 serves as an important biomarker for studying epigenetic mechanisms in development and disease. NSJ Bioreagents provides a validated PHF8 antibody optimized for its applications, supporting research into histone demethylation, transcriptional control, and developmental regulation.