Anti-PHACTR1 / Phosphatase and actin regulator 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PHACTR1 antibody detects Phosphatase and actin regulator 1, encoded by the PHACTR1 gene on chromosome 6p24.1. PHACTR1 antibody is applied in research on cytoskeletal regulation, actin remodeling, and cardiovascular disease. PHACTR1 belongs to the PHACTR family of proteins, which bind actin and protein phosphatase 1 (PP1). This dual binding capacity allows PHACTR1 to coordinate cytoskeletal dynamics with phosphatase-mediated signaling. Expression is widespread but particularly enriched in brain, vascular tissues, and heart, where it influences synaptic plasticity, endothelial function, and vascular remodeling.Structurally, PHACTR1 contains actin-binding RPEL motifs and PP1-binding sequences. These motifs enable it to tether PP1 to the cytoskeleton, coupling actin regulation with dephosphorylation of signaling proteins. This scaffolding function integrates actin remodeling with signaling cascades controlling cell shape, migration, and adhesion. PHACTR1 also contains nuclear localization sequences, suggesting additional roles in nuclear signaling.Functionally, PHACTR1 regulates endothelial cell function, neuronal differentiation, and vascular tone. In endothelial cells, PHACTR1 modulates angiogenesis and barrier integrity. In neurons, it influences spine morphogenesis and synaptic signaling. Genome-wide association studies have identified PHACTR1 polymorphisms associated with coronary artery disease, migraine, and cervical artery dissection. These findings establish PHACTR1 as a key link between cytoskeletal regulation and vascular biology. Researchers use PHACTR1 antibody to probe these pathways in cell and animal models.Clinically, PHACTR1 variants strongly influence cardiovascular disease risk. Polymorphisms in the PHACTR1 locus alter expression levels and endothelial function, predisposing individuals to coronary artery disease and aneurysm formation. Altered PHACTR1 expression has also been associated with neurological disorders, including migraine and epilepsy. By regulating both vascular and neuronal processes, PHACTR1 connects cytoskeletal control to common diseases. Its widespread associations make it an appealing biomarker and therapeutic target.Experimentally, PHACTR1 antibody is used in western blotting to detect the ~74 kDa phosphorylated protein, in immunohistochemistry to assess vascular expression, and in immunofluorescence microscopy to study actin cytoskeleton organization. Co-immunoprecipitation with PHACTR1 antibody helps reveal binding partners, including PP1 and actin. NSJ Bioreagents provides PHACTR1 antibody to support research into cytoskeletal regulation, cardiovascular biology, and neuronal function.