Anti-Peroxiredoxin 5 (PRDX5)

Peroxiredoxin 5 (Prx-5, also known as AOEB166 and thioredoxin reductase) is a 22kD, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54-63, and a peroxisome targeting motif at aa 208-210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H 2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17kD in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83-102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.