Anti-Peroxiredoxin 4 (PRDX4)

Peroxiredoxin 4 (Prx-IV, also known as PRDX4 and thioredoxin peroxidase AOE372) is a 24-26kD, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts p53-mediated generation of ROS, and regulates NFkappaB via IkappaB-alpha. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region (aa 20-30) and a 159 thioredoxin domain (aa 79-237). There is one catalytic cysteine at Cys124. Over amino acids 1-271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.