Anti-PEPD / Peptidase D, clone 30P28

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. PEPD antibody recognizes peptidase D, a cytosolic metallopeptidase responsible for hydrolyzing dipeptides with proline at the carboxyl terminus. This enzyme plays an important role in collagen metabolism, recycling proline-containing peptides into free amino acids for cellular reuse. PEPD is expressed widely in human tissues, particularly in fibroblasts and connective tissues, reflecting its role in extracellular matrix maintenance.Research using PEPD antibody has revealed multiple functional roles beyond collagen turnover. PEPD acts as a ligand for epidermal growth factor receptor (EGFR), activating signaling cascades that regulate cell growth and survival. This dual enzymatic and signaling capacity highlights PEPD as a multifunctional protein. In cancer biology, PEPD has been linked to tumor progression, where altered expression can support both metabolic adaptation and proliferative signaling. Elevated levels of PEPD have been reported in several tumor types, suggesting its potential as a biomarker.In hereditary diseases, mutations in PEPD cause prolidase deficiency, a rare autosomal disorder characterized by impaired collagen recycling, chronic skin ulcers, developmental delay, and immune dysfunction. Detecting PEPD levels with antibodies is important for research into this disorder as well as related connective tissue diseases. PEPD has also been associated with cardiovascular disease, where its enzymatic activity influences extracellular matrix remodeling and vascular health.Validated antibodies against PEPD are applied in western blot, immunohistochemistry, ELISA, and immunofluorescence. These reagents enable quantification of protein expression, detection of tissue-specific localization, and evaluation of signaling roles in EGFR-related pathways. Clone-based PEPD antibodies provide consistency required for both mechanistic studies and biomarker research.NSJ Bioreagents provides this PEPD antibody for applications in metabolism, connective tissue biology, oncology, and rare disease research.