Anti-PDF, ID (PDF, PDF1A, Peptide deformylase, mitochondrial, Polypeptide deformylase)

Anti-PDF, ID (PDF, PDF1A, Peptide deformylase, mitochondrial, Polypeptide deformylase)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
039814.200 200 µl - -

1 - 19 business days

557.00€
 
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase... more
Product information "Anti-PDF, ID (PDF, PDF1A, Peptide deformylase, mitochondrial, Polypeptide deformylase)"
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. Applications: Suitable for use in Western Blot, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-PDF, Anti-PDF1A, EC=3.5.1.88, Anti-Polypeptide deformylase, Anti-Peptide deformylase, mitochondrial
Supplier: United States Biological
Supplier-Nr: 039814

Properties

Application: ELISA, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: PDF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human PDF.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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