Anti-PCNA / Proliferating cell nuclear antigen, clone PCNA/13725R

1 mg/ml in 1X PBS, BSA free, sodium azide free. PCNA Proliferation Marker antibody, also known as Proliferating Cell Nuclear Antigen antibody, recognizes a nuclear replication factor commonly referred to as PCNA and Cyclin. PCNA is encoded by the PCNA gene located on chromosome 20p12 and is a highly conserved protein essential for DNA synthesis and genome maintenance. The protein localizes to the nucleus, where it forms discrete replication foci during S phase and serves as a central coordinator of DNA replication and repair. PCNA expression is tightly linked to cell cycle progression and is markedly elevated in actively dividing cells, making it one of the most widely used biomarkers of cellular proliferation.As a DNA sliding clamp, PCNA forms a homotrimeric ring that encircles double-stranded DNA and enhances the processivity of DNA polymerase delta and epsilon. By anchoring polymerases to the replication fork, it ensures efficient and continuous DNA synthesis. Beyond replication, PCNA functions as a molecular scaffold that recruits proteins involved in chromatin assembly, cell cycle regulation, and DNA damage response pathways. Through interactions with numerous PCNA-interacting protein motifs, it integrates replication with repair processes such as base excision repair, mismatch repair, and translesion synthesis.PCNA activity is dynamically regulated by post-translational modifications including ubiquitination, phosphorylation, and sumoylation. These modifications alter binding partner selection and help determine whether a cell engages high-fidelity repair pathways or damage tolerance mechanisms. The nuclear distribution pattern of PCNA changes throughout the cell cycle, reflecting its role in coordinating DNA synthesis and checkpoint control. This dynamic regulation underlies its value as a proliferation marker in both experimental and clinical contexts.Elevated PCNA expression is observed in a wide range of malignancies, including breast, colorectal, lung, prostate, and hematologic cancers. Strong nuclear staining correlates with increased growth fraction and is commonly used to assess tumor proliferative index. In non-neoplastic tissues, PCNA expression highlights regenerating or developmentally active cell populations, supporting its use in studies of tissue renewal and stem cell biology.PCNA Proliferation Marker antibody supports investigation of cell cycle kinetics, DNA replication dynamics, and tumor growth biology. Recombinant monoclonal clone PCNA/13725R recognizes PCNA and is suitable for detecting proliferating cells in relevant research applications.