Anti-Paxillin

The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the , plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the , integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role , in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased , Paxillin protein levels have also been reported in several malignancies, including renal, lung, and , breast carcinomas. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunocytochemistry: 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.