Anti-PARP16 / Poly ADP-ribose polymerase 16

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PARP16 antibody detects Poly(ADP-ribose) polymerase 16, a membrane-associated mono-ADP-ribosyltransferase that regulates the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress signaling. PARP16 belongs to the PARP family of enzymes involved in ADP-ribosylation, a post-translational modification that controls protein stability and signaling. The PARP16 antibody is commonly used in studies of ER stress, apoptosis, and ADP-ribosylation-dependent signaling pathways.PARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.The PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.Beyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target. NSJ Bioreagents provides a validated PARP16 antibody optimized for immunofluorescence, immunohistochemistry and flow cytometry assays, enabling precise study of ADP-ribosylation mechanisms and ER homeostasis.