Anti-PARP15 / Poly ADP-ribose polymerase 15

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PARP15 antibody recognizes Poly (ADP-ribose) polymerase 15, an enzyme belonging to the PARP family involved in ADP-ribosylation, a post-translational modification regulating transcription, DNA repair, and stress responses. Unlike the classical DNA repair PARPs, PARP15 functions primarily in transcriptional repression and RNA processing. It possesses both mono-ADP-ribosyltransferase and macro domains, allowing it to modify specific target proteins. The PARP15 antibody is an essential reagent for studies of ADP-ribosylation signaling, chromatin dynamics, and cellular stress adaptation.PARP15 is encoded by the PARP15 gene located on human chromosome 3q21.1. The protein comprises two N-terminal macro domains, a WWE domain involved in protein?protein interactions, and a C-terminal catalytic PARP domain. PARP15 localizes mainly to the nucleus and cytoplasmic granules, where it may regulate mRNA metabolism and response to stress. It has been implicated in cell survival pathways, as well as in the regulation of transcription factors under conditions of nutrient deprivation or oxidative stress.Research using the PARP15 antibody has demonstrated its involvement in cancer cell proliferation and inflammation. Aberrant expression of PARP15 has been observed in lymphomas and certain solid tumors, where it may contribute to altered transcriptional control and resistance to apoptosis. Western blot analysis typically detects a band at approximately 74?78 kDa. Immunocytochemical staining shows both nuclear and cytoplasmic localization, depending on cell type and stress conditions. Functional studies suggest that PARP15 can auto-modify and regulate its own enzymatic activity, similar to other PARP family members.In recent years, PARP15 has attracted attention as part of the broader ADP-ribosylation signaling network, which includes enzymes, hydrolases, and binding proteins that control protein modification during stress responses. The antibody supports exploration of this enzyme's role in RNA granule formation, viral defense, and oncogenic signaling pathways. NSJ Bioreagents provides a validated PARP15 antibody for applications such as western blot, immunohistochemistry, and immunofluorescence, ensuring reliable results across molecular biology and cancer research contexts.