Anti-Palladin (PALLD, Cytoskeletal associated protein, CGI-151, FLJ22190, FLJ38193, FLJ39139, KIAA09

Palladin (a cytoarchitectural protein named after the 16 century Venetian architect Palladio) is a cytoplasmic member of the myotilin/palladin family of proteins. Palladin is widely expressed and interacts with actin-associated proteins, thus promoting actin bundle stability. Human Palladin is 1383 amino acids (aa) in length and contains five I-type Ig-like domains (aa 271-360, 440-539, 1001-1085, 1135-1226, 1233-1324), a Pro-rich region (aa 634-864) and nine potential Ser/Thr phosphorylation sites. Multiple isoform variants exist. Predominant are a 140kD and 92-95kD short form that show alternative start sites at Met383 and Met712, respectively. The 140kD isoform also shows a deletion of aa 656-879 to generate an approximately 115kD isoform. Apoplications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.