Anti-p73, alpha, beta, gamma, delta (Tumor Protein p73, p53-like Transcription Factor, p53-related P

p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% aa identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker/ Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer. Applications: Suitable for use in ChIP, Immunofluorescence, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: p73 transfected cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.